Coding

Part:BBa_K4389012

Designed by: Arsen Orazbek   Group: iGEM22_NU_Kazakhstan   (2022-10-08)


B5R sequence with altered Cys140 to Ser

The B5R protein altered by site mutagenesis can be important for proper protein folding. The Cys140 does not form disulfide bridges. However, recombinant protein can be folded improperly due to the close presence of this Cys to another Cys residue. Thus, we suggested changing this site on the pET+B5R construct that our team prepared (BBa_K4389000).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 279
    Illegal BamHI site found at 165
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 279
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 656


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Categories
Parameters
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