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Coding
Part:BBa_K4389012
Designed by: Arsen Orazbek Group: iGEM22_NU_Kazakhstan (2022-10-08)
B5R sequence with altered Cys140 to Ser
The B5R protein altered by site mutagenesis can be important for proper protein folding. The Cys140 does not form disulfide bridges. However, recombinant protein can be folded improperly due to the close presence of this Cys to another Cys residue. Thus, we suggested changing this site on the pET+B5R construct that our team prepared (BBa_K4389000).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 279
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 279
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 279
Illegal BamHI site found at 165 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 279
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 279
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 656
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Categories
Parameters
None |